The Johnstone River, comprising the North Johnstone River and the South Johnstone River, is a river system in Far North and North Queensland, Australia. The headwaters of the river system rise in the Atherton Tablelands.
The north branch of the river system rises below Merivale, flows over the Malanda Falls and through the town of Malanda and then flows generally south by east, around Francis Range and over the Jones Falls, before flowing east, covering a distance of 114 kilometres (71 mi). The south branch of the river system rises below Mount Father Clancy, southwest of Mungalli, and generally flows east over Binda Falls, through the settlement of South Johnstone, before flowing north, covering a distance of 88 kilometres (55 mi).